FastQCFastQC Report
Sun 19 Mar 2023
SRR4895739_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895739_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423136
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG52941.2511343870528624No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49471.1691276563563489No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA7640.18055660591393782No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5480.1295091885351282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5000.11816531800650382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4840.11438402783029569No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGA203.864649E-4108.74893145
AAGCAGT107000.0105.983451
AGTGGTA108300.0104.10875
GGTATCA109000.0103.49448
AGCAGTG109550.0103.3840942
GTGGTAT109350.0102.765346
TGGTATC109600.0102.597087
GTATCAA110400.0102.181979
CAGTGGT110850.0101.7137764
GCAGTGG111150.0101.439253
CGACTGG1109.9325916E-846.1413651
AGGGGTA750.001481628338.670865
AGTGATA2207.4617674E-726.3664935
GGGTATC1100.00966586226.3633797
CCGATTA300.001494908524.1664345-49
ATGGGAG18950.023.10795225-29
CATGGGG18200.022.30747225-29
TAAGGTT2303.1367366E-522.065002145
GCACATC1650.002598551321.969482145
ACATGGG104250.021.87844320-24
Produced by FastQC (version 0.11.5)