FastQCFastQC Report
Sun 19 Mar 2023
SRR4895738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673094
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT673700.0144.070721
GAACTTA674300.0143.942542
AACTTAG227050.0143.415923
ACTTAGG81850.0142.095614
ACTTATG86600.0142.045064
ACTTAGA64950.0141.989564
ACTTAGT79950.0141.504244
AACTTAT240700.0141.274143
CTTATAG26750.0140.70575
ACTTAAG71500.0140.37774
AACTTAA207400.0139.23833
CTTAGGA24350.0138.287495
CTTAGTG25000.0137.576225
CTTAGGT27550.0136.88165
ACTTATA72450.0136.845114
CTTAGAG21200.0136.727285
CTTATTG35300.0136.652135
CTTAGTA22900.0136.023285
CTTATGG31550.0135.982365
ACTTACG3500.0135.96934
Produced by FastQC (version 0.11.5)