FastQCFastQC Report
Sun 19 Mar 2023
SRR4895738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673094
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83141.2351915185694717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69281.029276742921494No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13830.20546907267038483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9450.1403964379418031No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT8350.12405399543005881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7610.11305998864943084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128200.099.244138
AGTGGTA128450.099.171225
GTGGTAT128400.098.86376
AAGCAGT129950.098.535941
GTATCAA129700.098.040479
TGGTATC129500.098.0239267
AGCAGTG132550.096.603142
CAGTGGT133200.095.532954
GCAGTGG133950.095.1604463
CGAACTT753.7568134E-758.0056461
CGGTCTG2100.051.78306145
CGATAGG353.7769933E-629.001135-39
CCGCAAG7600.025.755259145
GTCGGTA401.0667467E-525.37407790-94
TGCGGCT4700.024.679585145
CATGGGG37950.024.30131725-29
GGTCTGA3102.4161636E-823.385897145
TACGCAC1650.022.84731350-54
ATAGGCG2800.021.750824100-104
CGACCTA1750.021.54175445-49
Produced by FastQC (version 0.11.5)