FastQCFastQC Report
Sun 19 Mar 2023
SRR4895737_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895737_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208648
Sequences flagged as poor quality0
Sequence length151
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT28270.23389771049966576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTGA1211100.0143.901322
TCCTGAG424000.0143.637833
TCCTGAT413350.0143.536163
CCTGATG148000.0143.029824
TCCTGAA354700.0142.83713
CCTGAGT158500.0142.788834
CCTCCTG1221750.0142.646941
CCTGATA123950.0142.33764
CCTGAGA118650.0142.19374
CTGAGTA47750.0142.085085
CTGATAG44450.0141.928155
CCTGATT140200.0141.627694
CCTGAAG125200.0141.607564
CCTGAAT130400.0141.489734
CTGATTG50900.0141.224355
CCTGAGG145600.0141.07934
CTGAGTG50500.0140.915255
CTGAGAG41950.0140.76165
CTGATGG51700.0140.712555
CTGAATG47200.0140.22745
Produced by FastQC (version 0.11.5)