FastQCFastQC Report
Sun 19 Mar 2023
SRR4895737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208648
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG172551.4276282259185469No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102640.8492133358926669No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA24070.19914813907771328No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18000.14892673466551054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA242650.088.354868
GTGGTAT242350.088.075326
AAGCAGT244100.087.919131
AGTGGTA243800.087.759665
GTATCAA244650.087.506799
TGGTATC245050.087.1344767
CAGTGGT248150.086.2868354
AGCAGTG249650.085.881012
GCAGTGG253600.084.403893
CGGTCTG2800.038.839596145
CGGAGCC5400.028.194672145
CGATAGG2800.024.34008435-39
TACGCAC3050.022.81834450-54
CATGGGG66800.022.55239125-29
CGACCTA3250.021.41431445-49
ATGGGGT14300.020.7860225-29
ACGCGCT2100.020.02330830-34
ATAGGCG3350.019.91143100-104
CGGCTAT2900.019.500317140-144
TACATGG231950.019.15894720-24
Produced by FastQC (version 0.11.5)