FastQCFastQC Report
Sun 19 Mar 2023
SRR4895736_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895736_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253197
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA253250.0144.137022
CCGTGAG253500.0143.994871
GTGAGAG82450.0142.794863
GTGAGAT87500.0142.710823
TGAGATG32300.0141.738654
GTGAGAA81250.0141.620643
TGAGAGT27600.0141.581764
TGAGAGA25250.0140.767564
TGAGATA26950.0139.64614
TGAGAGG29550.0137.606414
TGAGAAG30500.0137.338844
TGAGAAT28000.0136.726784
TGAGATT29250.0136.306434
TGAGAAA26200.0130.4354
GTGAGAC5650.0130.157363
GAGAATG10550.0129.159525
GAGATGA10500.0129.087925
GAGATGG11750.0128.854175
GAGATAG9250.0127.046565
AGAATCG800.0126.1697166
Produced by FastQC (version 0.11.5)