FastQCFastQC Report
Sun 19 Mar 2023
SRR4895736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253197
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG59052.332176131628732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19780.7812098879528588No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG16710.6599604260713989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA9340.368882727678448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC8400.3317574852782616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG3850.1520555140858699No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGGGCGCAGTGGCTCACG3550.14020703246878913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT3300.1303332977878885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTCG100.0070879594144.998612
AAGCAGT91950.0101.554221
GGTATCA93600.098.8345268
CAGTGGT93800.098.701094
GTATCAA94350.098.202559
GTGGTAT94300.098.023996
TGGTATC94500.097.816537
AGCAGTG95150.097.681672
AGTGGTA95300.097.147555
GCAGTGG98200.094.204813
CGTGAGA701.6924532E-551.785222
TAGTATC752.5452851E-548.332877
CCGTGAG803.726915E-545.3120651
AAATATA8850.045.056065145
CAGTAGT1652.377321E-630.7572844
AGTGATA1804.682126E-628.1941765
GCAGTAG1306.469841E-427.884353
AAACCGC3800.027.47342185-89
GCATTGA3900.026.39718430-34
CGCAGAT4100.025.81682890-94
Produced by FastQC (version 0.11.5)