FastQCFastQC Report
Sun 19 Mar 2023
SRR4895735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213834
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAGT1214900.0144.069471
CGAAGTA1214950.0144.063552
GAAGTAG398400.0142.842733
GAAGTAT437050.0142.616323
GAAGTAA371500.0142.278663
AAGTATG158950.0139.482574
AAGTAGG144200.0138.750034
AAGTAGT145250.0137.84634
AAGTAAG132350.0136.51884
AAGTAGA119500.0136.296174
AAGTATA142400.0135.440694
AAGTAAT135100.0134.060134
GAAGTAC22000.0133.385043
AGTATGA47950.0132.621925
AGTATAG50700.0131.685645
AAGTATT156100.0130.805424
AGTAGTA49350.0130.17455
AGTATTG57800.0130.104355
AGTAGGT50450.0129.622835
AGTAGAG38700.0128.923025
Produced by FastQC (version 0.11.5)