FastQCFastQC Report
Sun 19 Mar 2023
SRR4895735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1213834
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG100940.8315799359714755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98420.8108192718279437No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23780.1959081719576153No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT16450.13552100204805598No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15190.12514066997629No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC14810.12201009363718598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193650.0101.4913568
GTGGTAT193450.0101.4838646
GTATCAA195300.0100.782399
AAGCAGT196150.0100.5553051
AGTGGTA196450.099.90135
TGGTATC196800.099.645847
AGCAGTG200200.098.331892
CAGTGGT201550.097.421424
GCAGTGG205950.095.199243
CGGTCTG3950.051.39204145
CGAAGTA2057.2759576E-1238.9069752
CGACCTA4050.025.41894345-49
TACGCAC4150.025.15644650-54
CCGAAGT3251.4824764E-924.5433431
CATGGGG59400.023.55564925-29
CGATAGG502.2817112E-623.2007935-39
CGGCGGT5000.021.75074235-39
GTCGGTA1400.021.7489590-94
TACCTCG3700.021.55478935-39
CGCGAAA3650.021.45101790-94
Produced by FastQC (version 0.11.5)