FastQCFastQC Report
Sun 19 Mar 2023
SRR4895734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816520
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49410.6051290844069955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCA818000.0143.930632
CCAGTTC818250.0143.886661
AGTTCAG277000.0143.518313
AGTTCAT294050.0143.312383
AGTTCAA233850.0142.807663
GTTCATG110800.0142.76354
GTTCAGT104900.0142.201874
GTTCAGA77150.0142.045654
GTTCAGG94600.0141.832444
GTTCAAT83650.0141.006034
GTTCATA87050.0140.964944
GTTCAAG86300.0140.853334
GTTCATT98250.0138.691424
TTCAGTA31600.0136.894015
TTCAGTG32800.0136.281895
TTCAGAT30450.0135.434425
AGTTCAC18050.0135.407533
TTCAGAG23800.0135.107125
TTCAGGA28050.0134.427835
GTTCAAA68550.0134.20344
Produced by FastQC (version 0.11.5)