FastQCFastQC Report
Sun 19 Mar 2023
SRR4895734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816520
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG186032.2783275363738795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72370.8863224415813452No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29480.36104443246950474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC15280.18713564885122227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG14880.18223680987605936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10610.12994170381619555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT200450.081.376651
GTGGTAT205000.079.261916
GGTATCA206250.079.238558
AGTGGTA205750.079.1139455
GTATCAA207850.078.6538249
TGGTATC207050.078.617217
AGCAGTG208850.078.0437852
CAGTGGT209050.077.761024
GCAGTGG212200.076.60673
CCGCAAG8450.032.604446145
CGATAGG1450.024.99915335-39
CGGTCTG2951.4266334E-824.576946145
CATGGGG42050.023.8267825-29
CATGCGA1550.001807917623.387737145
CGACCTA2550.022.7443345-49
CTCGTTG1500.022.2325835-39
TACGCAC2650.021.88712550-54
CGCGAAA1550.021.51540290-94
TGCGGCT5400.021.48207145
CGCAAGG5453.6379788E-1219.954676145
Produced by FastQC (version 0.11.5)