FastQCFastQC Report
Sun 19 Mar 2023
SRR4895732_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895732_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576134
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAT151.2575171E-4144.189126
ACTTCGA576400.0144.114072
CTTCGAG186400.0144.03443
CACTTCG577100.0143.939271
CTTCGAT209750.0143.398573
CTTCGAA171850.0143.266173
CGATGAG6750.0143.121056
TTCGAGA56650.0142.789224
TTCGATA64750.0142.741654
TCGAGAG18800.0142.65525
TTCGAAT64000.0142.612064
TCGATAG21650.0142.524125
TCGATTA21000.0142.472585
TTCGATG73700.0142.428334
TTCGAGG62400.0142.340534
TTCGAAG57500.0142.308384
TTCGAGT67350.0142.048224
TCGATTG25550.0141.931755
TCGAAGT20250.0141.696965
TCGATAA20900.0141.429525
Produced by FastQC (version 0.11.5)