FastQCFastQC Report
Sun 19 Mar 2023
SRR4895732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576134
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG52850.9173213176101394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45350.7871432687534498No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8340.1447579903286388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8260.14336942447416748No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5840.10136530737640896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT136900.0110.995291
GGTATCA137550.0110.164098
TGGTATC137550.0109.795127
GTGGTAT137500.0109.624146
AGTGGTA138450.0109.1337665
GTATCAA139700.0108.7706159
CAGTGGT139850.0108.196784
AGCAGTG142300.0106.4358442
GCAGTGG145000.0104.203933
CGGTCTG1300.061.34314145
CGTTATC450.00963564148.335163
CACTTCG1851.6422746E-731.3498121
CATGGGG30650.024.45720325-29
GCCCCAA4500.024.165482145
CGACCTA2100.023.47626145-49
ACATGGG130050.023.16764320-24
CAACGCA139050.023.03410710-14
CGCGAAA1200.022.95720790-94
ATCAACG138650.022.95415710-14
TACGCAC2150.022.92910650-54
Produced by FastQC (version 0.11.5)