FastQCFastQC Report
Sun 19 Mar 2023
SRR4895731_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895731_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124855
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1127250.0143.892211
AATGGAA1139600.0142.332842
ATGGAAG373900.0141.653853
ATGGAAT428850.0141.610143
TGGAATG151200.0139.812234
TGGAAGT147700.0139.122484
ATGGAAA332200.0138.968633
TGGAATA128700.0138.3734
TGGAAGG136800.0136.715214
TGGAAGA97750.0135.938194
TGGAAAG121150.0135.867464
TGGAAAT130500.0135.580434
TGGAATT156800.0135.186774
GGAATTG52350.0133.869775
GGAATAG44900.0132.316445
GGAAGTA48950.0132.268555
GGAATGA44050.0131.268775
GGAATGT56300.0130.112275
GGAAGTG49350.0127.836195
GGAAGGT49300.0126.7958765
Produced by FastQC (version 0.11.5)