FastQCFastQC Report
Sun 19 Mar 2023
SRR4895731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124855
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG124291.1049424148001297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123791.1004973974423369No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17330.15406430162109783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194650.088.868028
AGTGGTA195800.088.053775
AAGCAGT197400.088.0535661
GTGGTAT195900.087.96796
GTATCAA198550.087.4471749
TGGTATC198350.086.881337
AGCAGTG202350.085.461912
CAGTGGT203000.085.045394
GCAGTGG209350.082.292623
CGGTCTG2850.043.248615145
CCGACGC750.001482960138.669346145
GCGAACG200.00607871628.99814235-39
CAATGGA8550.025.4437541
TACGCAC3700.024.68760950-54
CGACCTA3700.023.51200945-49
CATGGGG58100.022.71140725-29
CGCGAAA2400.022.35352990-94
GCCCCAA10950.021.850828145
TGACGAC4200.020.36774340-44
CGGCGGT4700.020.05190735-39
Produced by FastQC (version 0.11.5)