FastQCFastQC Report
Sun 19 Mar 2023
SRR4895730_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895730_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866955
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT150861.740113385354488No Hit
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT26710.30808980858291374No Hit
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT9870.11384673945014448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCTA867850.0144.03861
TGCCTAA868400.0143.947372
GCCTAAG328400.0143.683213
GCCTAAT297200.0143.557423
GCCTAAA234250.0143.049413
CCTAAGT107900.0142.851824
CCTAATG150150.0142.507614
CTAAGTG41100.0142.4345
CCTAAGG129100.0141.954384
CCTAAAG100400.0141.459474
CTAAGGT41450.0141.057395
CTAATGT69400.0140.86395
CTAAGAG34350.0140.620155
CTAATGG46800.0140.4915
CCTAAGA94200.0140.131884
CTAATAG32100.0139.920875
CTAAATG35000.0139.65655
CTAAGTA30600.0139.47615
CCTAAAT77600.0138.706744
CTAATGA30350.0138.487125
Produced by FastQC (version 0.11.5)