FastQCFastQC Report
Sun 19 Mar 2023
SRR4895730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866955
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG288283.325201423372609No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67050.77339654307317No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA56610.6529750679100992No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23450.27048693415459857No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT18120.209007387926709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12760.14718180297708647No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11310.13045659809332663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT251050.070.3209461
AGCAGTG257350.068.47442
GGTATCA265300.066.336668
GTATCAA266400.065.993089
GTGGTAT266350.065.857366
TGGTATC267100.065.726727
AGTGGTA268050.065.5208365
CAGTGGT271750.064.659154
CGGTCTG2250.064.4419145
GCAGTGG280500.062.56463
AGCGCAT1153.1624862E-431.520496145
CCGTCTT8550.027.133434145
CCGCAAG8150.026.686066145
CGACCTA2900.024.99901645-49
CATTCGG2351.3305525E-624.67988145
CCGTCGT1801.5204641E-424.165716145
GCCCCAA8000.023.561573145
TACGCAC3150.023.47526650-54
TGCGGCT4455.456968E-1222.80809145
TCTCGCA3650.021.05396570-74
Produced by FastQC (version 0.11.5)