FastQCFastQC Report
Sun 19 Mar 2023
SRR4895729_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895729_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932997
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGACTATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC16020.1717047321695568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT934050.0144.033621
AAGACTA934550.0143.956562
AGACTAG323750.0143.869383
AGACTAT332450.0143.747993
GACTAGT117750.0143.652344
GACTATG128400.0143.585754
AGACTAA265150.0143.496413
GACTAGG117300.0143.281424
GACTAAT94850.0143.215234
GACTAGA86800.0142.957444
GACTAAG96700.0142.935884
GACTATA92400.0142.720834
ACTAGAG30700.0142.324575
GACTATT111200.0142.258264
ACTAGTG43400.0142.043725
ACTAGTA32350.0140.860245
ACTATAG33500.0140.759775
ACTAGGT39400.0140.726465
ACTAATG33850.0140.582375
ACTATGA32850.0140.252665
Produced by FastQC (version 0.11.5)