FastQCFastQC Report
Sun 19 Mar 2023
SRR4895729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932997
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG240002.5723555381207013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73740.7903562390875856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29830.31972235709225216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20910.2241164762587661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT16380.17556326547673787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC13730.14716017307665513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCACGGTGGCTCA11220.12025762140714279No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10770.11543445477316648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT256250.080.0125051
GGTATCA258700.079.174938
GTATCAA260600.078.732569
GTGGTAT259950.078.487396
AGTGGTA260800.078.314995
TGGTATC261700.078.0733647
CAGTGGT261850.078.032824
AGCAGTG264850.077.199552
GCAGTGG270650.075.388473
CCGCAAG8400.026.754786145
CGCAAGG5100.022.744104145
CATGGGG56550.022.71841825-29
TACGCAC2500.022.61901350-54
CGACCTA2600.022.30671945-49
GAGGTAT2755.207181E-621.092256
TGCGGCT4251.4697434E-920.469694145
ATGGGGG22750.020.33228925-29
TTAGCGC659.849682E-720.07604840-44
AGGTATC2908.243982E-620.0012727
ATGGGGC19700.019.87350725-29
Produced by FastQC (version 0.11.5)