FastQCFastQC Report
Sun 19 Mar 2023
SRR4895728_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895728_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957871
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT354123.6969487540597847No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT63440.6623021262779644No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT37600.3925371996855527No Hit
CAACCACATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT11800.12318986585876386No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT11770.12287667128454667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGT128000.0142.816855
ACCACAT407100.0142.648773
CCACATG198750.0142.592564
ACATGTA105250.0142.375386
CAACCAC971450.0142.206191
AACCACA976000.0141.543232
CCACAGT105100.0140.931854
CACATAG33450.0139.05135
CACAGTA32650.0138.482795
CACAGAT33100.0138.3435
CCACATA101550.0138.26034
CACATTG38500.0138.044225
CCACAAT102550.0137.123034
CACAATG34850.0136.982995
CCACATT110750.0136.932654
CACATGA29600.0136.916585
CCACAGA90000.0136.853784
CCACAAG85200.0136.777044
CACAGGT30400.0136.634495
CACAGTG33950.0136.57865
Produced by FastQC (version 0.11.5)