FastQCFastQC Report
Sun 19 Mar 2023
SRR4895728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957871
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG674987.046669123504104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA106931.116329860701493No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80840.8439549793239381No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA15780.16474034603824525No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA12610.13164611936262816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGGTTGAGATCGGAAGAGCACA10670.11139287022991615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT481100.054.302021
AGCAGTG498700.052.3537982
AGTGGTA498900.052.1847535
GTGGTAT502800.051.760166
GGTATCA505750.051.7305838
TGGTATC505450.051.589187
GTATCAA507300.051.5439539
CAGTGGT506550.051.4422764
GCAGTGG518650.050.242143
CATGCGT1451.9284926E-839.998257145
CGGTCTG3650.037.738083145
CATGCGG9750.032.716522145
CGGGCTC4350.029.998693145
CGTGGGG5300.028.725163145
CCGGCTT6250.027.838787145
CGTGCGG3850.026.362486145
CGACCTA3000.025.61421445-49
CGTGGGA6350.025.117016145
CCGTCTT16550.023.65456145
TACGCAC3350.023.37089750-54
Produced by FastQC (version 0.11.5)