FastQCFastQC Report
Sun 19 Mar 2023
SRR4895727_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895727_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174008
Sequences flagged as poor quality0
Sequence length151
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGGA1180500.0143.408652
ATTGAGG1181100.0143.33581
TGAGGAT442500.0142.736183
TGAGGAG391400.0142.121033
TGAGGAA339050.0141.927223
GAGGATG153450.0136.543784
GAGGATA143150.0136.446014
GAGGAGT149750.0135.439744
GAGGATT167050.0133.412254
GAGGAAT137700.0133.102084
AGGATGA42650.0131.185525
AGGATAG48600.0130.553835
AGGATTG53950.0130.169945
AGGAGTA44150.0129.994695
GAGGAAG117850.0129.274584
GAGGAGA123800.0128.7694
AGGATGT56950.0128.503665
AGGATGG56300.0128.194355
AGGATTA50150.0127.525175
AGGATAT52950.0127.453895
Produced by FastQC (version 0.11.5)