FastQCFastQC Report
Sun 19 Mar 2023
SRR4895727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174008
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA117360.9996524725555532No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG68720.5853452446661351No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG12400.10562108605733521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT140500.0103.820026
GGTATCA141750.0103.7228248
AGTGGTA141700.0103.145475
GTATCAA142600.0102.90129
TGGTATC144050.0101.4124457
AAGCAGT147200.099.5462651
CAGTGGT146950.099.518264
AGCAGTG147450.099.328312
GCAGTGG153600.095.162493
CGGTCTG3100.037.420216145
TACGCAC2100.024.16619350-54
CATGGGG51950.022.94276225-29
CGTCGGT1100.022.40922290-94
TACATGG136700.021.89276520-24
TCAACGC141450.021.87514510-14
CAACGCA141900.021.82620810-14
ATCAACG140700.021.81655710-14
GTACATG140400.021.72891820-24
AGAGTAC142850.021.58970515-19
AACGCAG143800.021.48740810-14
Produced by FastQC (version 0.11.5)