FastQCFastQC Report
Sun 19 Mar 2023
SRR4895726_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895726_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577912
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22650.3919281828375254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTA578800.0143.971152
ATCCTGT579200.0143.871721
CCTGTAG183000.0143.72263
CTGTAGT71850.0143.191914
CCTGTAA164850.0143.189453
CTGTAAG53950.0143.126274
CTGTATG72450.0143.001194
CCTGTAT214250.0142.950273
CTGTAGA51400.0142.792684
CTGTAGG59350.0142.616134
CTGTAAT64200.0141.949334
CTGTATA62800.0141.210424
CTGTATT79400.0139.20124
CTGTAAA48400.0137.492084
TGTAGTA22000.0133.0535
TGTAGTG24450.0132.989995
CTGTACT13600.0132.53254
TGTAATG21950.0132.042225
TGTATGA20000.0131.938775
CCTGTAC21300.0131.332863
Produced by FastQC (version 0.11.5)