FastQCFastQC Report
Sun 19 Mar 2023
SRR4895726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577912
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG176733.058078046484586No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG52200.9032517061421117No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50470.8733163526626891No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA33940.5872866457176871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC18230.3154459502484807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA8180.14154404130732706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7640.1322000581403397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT222250.083.156441
GTATCAA226350.081.386889
GTGGTAT227450.080.745266
AGCAGTG229150.080.532892
TGGTATC228800.080.3322147
CAGTGGT229550.080.203034
GGTATCA229850.080.0283368
GCAGTGG232900.079.0805363
AGTGGTA234850.078.262775
AAATATA20600.048.215157145
CCGCAAG4300.030.348293145
CGCAAGG2951.4551915E-1129.491145
TGCGGCT1358.079406E-426.851372145
AAACCGC10150.025.71382585-89
CGCAGAT10950.024.4972890-94
GAAACCG10900.024.34360185-89
GCGCAGG10850.024.18850745-49
AGCGCAG11100.023.90497845-49
GCATTGA11500.022.94741630-34
CCAGCGC11650.022.77641945-49
Produced by FastQC (version 0.11.5)