FastQCFastQC Report
Sun 19 Mar 2023
SRR4895725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415501
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA100.007209415144.187967
GTCACTA415900.0144.049292
AGTCACT415950.0144.031971
TCACTAG117950.0143.76013
TCACTAA185850.0143.489733
CACTAAG55600.0143.409974
TCACTAT106750.0143.242463
CACTAGA68200.0143.130874
CACTAGG35800.0142.979694
CACTATG34250.0142.9254
CACTATA63000.0142.013694
CTATAAG12650.0141.338416
ACTATGA18600.0141.087165
CACTAAA109150.0140.687294
CACTAGT14100.0140.608844
ACTAAAG37250.0140.317155
ACTATAG24000.0139.982485
ACTAGGA21450.0139.818635
ACTAGAG17900.0139.757615
ACTAAGA35350.0139.701225
Produced by FastQC (version 0.11.5)