FastQCFastQC Report
Sun 19 Mar 2023
SRR4895725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415501
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG111172.6755651610946787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55511.3359775307399984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17640.42454771468660724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16540.39807365084560564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9850.23706320803078695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCCGGTGGCTCAC8190.19711143896163907No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA6270.15090216389370906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA6030.1451260045102178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA5990.14416331127963591No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCCGGCGCGGTGGGTCAG5920.14247859812611763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGAGTCTGGAAATAACT4570.1099877015939793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT183600.096.782311
GTGGTAT184400.095.536826
AGCAGTG186000.095.2216952
GGTATCA185750.095.15478
GTATCAA186950.094.815389
TGGTATC186350.094.5760047
CAGTGGT186700.094.437544
AGTGGTA188250.093.659965
GCAGTGG189100.093.238963
CGGTCTG701.6945462E-551.78421145
GAGGTAT1306.4776244E-427.8838066
GCCCCAA2903.947207E-1027.499203145
AGGTATC1358.076091E-426.8510727
ATACGCC1650.026.366043110-114
AGCGGAC1100.00966657526.36287145
GTGATAT2808.169081E-925.8921056
AGAGGTA1409.986426E-425.8921055
GATACGC1750.025.688059110-114
TAGTATC1701.0300756E-425.5874927
ATGGGAG28000.024.07965925-29
Produced by FastQC (version 0.11.5)