FastQCFastQC Report
Sun 19 Mar 2023
SRR4895724_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895724_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580640
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGGA582650.0143.685931
GCAGGAA582800.0143.648942
CAGGAAT211700.0143.37593
CAGGAAG182450.0142.612613
CAGGAAA183800.0139.917663
AGGAATG68250.0138.383274
AGGAAGT69750.0137.99144
AGGAATA69900.0137.901554
AGGAATT80600.0135.248264
AGGAAAT74200.0133.019244
GGAATAG23800.0132.076175
GGAATGA19700.0131.38425
GGAATGT26150.0129.856635
GGAATAT25000.0129.773935
GGAATTG28200.0129.10925
GGAAGTG23150.0127.687335
AGGAAAG66250.0127.3253564
GGAAGTA23050.0127.302945
AGGAAGG69800.0127.253644
AGGAAGA56350.0127.176664
Produced by FastQC (version 0.11.5)