FastQCFastQC Report
Sun 19 Mar 2023
SRR4895724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580640
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG125072.1540024800220445No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58831.0131923394874622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21140.3640810140534583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT6120.10540093689721686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT162950.084.358371
GGTATCA164400.083.878938
AGTGGTA163700.083.661855
GTATCAA165400.083.276969
GTGGTAT165450.082.776946
CAGTGGT166950.082.127144
AGCAGTG167750.081.821922
TGGTATC168500.081.321637
GCAGTGG170800.080.191023
CGGTCTG2050.042.43963145
CACGCGG750.001482769438.667217145
CGGAGCC2603.7125574E-927.885014145
TGCGGCT4000.027.187887145
GGTCTGA3151.031367E-925.317822145
CATGGGG31500.022.92216325-29
TACGCAC1700.022.17678650-54
GCCCCAA5251.8189894E-1220.714582145
CCCGCGA1650.020.21066790-94
CGGCGGT1950.020.0772135-39
CCGCAAG4001.6278136E-819.937784145
Produced by FastQC (version 0.11.5)