FastQCFastQC Report
Sun 19 Mar 2023
SRR4895723_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895723_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598063
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCA598550.0144.074262
AGATCGC598800.0144.014111
ATCGCAT216100.0143.861083
ATCGCAG201350.0143.765053
ATCGCAA168850.0143.554233
TCGCAAT66100.0143.540274
TCGCATA65550.0143.534794
TCGCAAG50850.0143.060444
TCGCAGT74950.0143.040394
TCGCATT77600.0142.986914
TCGCAGA55450.0142.89454
CGCATAG20300.0142.774085
TCGCATG72450.0142.602514
TCGCAGG69800.0142.542074
GCATGAT7800.0142.346076
CGCAAGT20000.0142.031785
CGCAGAT17750.0141.351445
CGCATTA21950.0141.238545
CGCATTG26700.0141.224415
CGCATAT21750.0141.211385
Produced by FastQC (version 0.11.5)