FastQCFastQC Report
Sun 19 Mar 2023
SRR4895723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598063
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79581.3306290474414904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65621.0972088224819125No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12370.20683439704512735No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA6760.11303157025263225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT175300.0109.224291
GGTATCA176700.0107.989648
AGTGGTA176500.0107.824475
GTGGTAT176450.0107.64966
TGGTATC177450.0107.165517
GTATCAA178650.0106.8920759
AGCAGTG179750.0106.1572652
CAGTGGT179550.0106.1140064
GCAGTGG183200.0103.801963
CGGTCTG1102.4351571E-432.954147145
AGGTATC1851.641929E-731.3509717
GAGGTAT1902.0744483E-730.5259466
AGGGGTA1409.990586E-425.8925445
CATGGGG30150.024.57680125-29
ATGGGAG28350.024.34701225-29
ATGGGAT37650.023.33992425-29
ACATGGG167300.022.98593120-24
GTGATAT4455.456968E-1222.8087125
TACATGG174400.022.56565320-24
TGATATC4505.456968E-1222.5552836
Produced by FastQC (version 0.11.5)