FastQCFastQC Report
Sun 19 Mar 2023
SRR4895722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506500
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCAA507300.0143.963352
AGAGTCA507300.0143.963351
AGTCAAT189800.0143.469013
AGTCAAG166000.0143.452413
GTCAATG66200.0142.883874
GTCAAGA51050.0142.778474
GTCAAGT55500.0142.5024
GTCAATA56500.0142.021474
AGTCAAA147550.0141.698783
GTCAAAG52300.0141.571584
GTCAATT66650.0141.486474
GTCAAGG59050.0141.38264
GTCAAAT51800.0141.128754
TCAATAG19400.0139.359595
TCAATGT22350.0137.416665
TCAAGGT21600.0136.847675
TCAATGG23800.0136.617685
TCAATTG23350.0135.85425
TCAAGAT17750.0135.661135
TCAAGTA18150.0135.054675
Produced by FastQC (version 0.11.5)