FastQCFastQC Report
Sun 19 Mar 2023
SRR4895722_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895722_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506500
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83331.6452122408687067No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50020.9875616979269497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12200.24086870681145114No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6810.13445212240868706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107950.087.111148
AAGCAGT108600.086.848231
GTGGTAT108250.086.534836
AGTGGTA108600.086.255945
TGGTATC109150.085.887737
GTATCAA109450.085.842579
CAGTGGT109850.085.274434
AGCAGTG110750.084.974242
GCAGTGG112600.083.12743
CGGTCTG2008.9276E-932.626377145
GCCCCAA5750.030.262148145
TACGCAC2300.025.21646750-54
CGTCGGT300.001494536924.16768695-99
CATGGGG23950.022.82334525-29
CGACCTA2550.022.74516145-49
TCCGGCG2150.022.25675435-39
TCTCGCA2900.021.49963670-74
TCTCGCG350.00365936220.7151635-39
GTCGGTA350.00365936220.7151695-99
CGGAGCC2104.308856E-420.715158145
Produced by FastQC (version 0.11.5)