FastQCFastQC Report
Sun 19 Mar 2023
SRR4895721_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895721_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666570
Sequences flagged as poor quality0
Sequence length151
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC666900.0144.124311
CGTATCA667250.0144.04872
GTATCAG224400.0143.975083
GTATCAA192900.0143.22823
GTATCAT242100.0143.09813
TATCAGA62950.0142.94014
TATCAGT79700.0142.933494
TATCAGG81400.0142.605644
TATCATG88650.0142.573364
TATCAAG65850.0141.791184
ATCATGA25400.0141.645265
ATCAGGT27700.0141.33685
ATCAGTA23500.0140.825095
ATCAGTG25400.0140.509845
ATCAGAT22850.0139.782495
ATCATGG30650.0139.730485
TATCAAT72750.0138.947344
TATCATA75600.0138.573144
ATCATAG25300.0138.215415
ATCAGGG27150.0137.82655
Produced by FastQC (version 0.11.5)