FastQCFastQC Report
Sun 19 Mar 2023
SRR4895721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666570
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG76641.1497667161738452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66731.0010951587980257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11780.17672562521565627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC8370.12556820739007155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7440.11161618434673028No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7190.10786564051787509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT120350.093.134266
AAGCAGT122000.092.741431
AGTGGTA121500.092.379025
GGTATCA121450.092.350428
AGCAGTG123400.091.2190552
GTATCAA123750.091.095889
TGGTATC123150.091.075597
CAGTGGT124650.090.1297454
GCAGTGG126450.088.7894063
CGGTCTG1450.054.996902145
ACGTATC1801.286844E-732.2349131
GCCCCAA8050.027.017109145
CGACCTA2850.024.92916745-49
TACGCAC3000.024.6486150-54
CATGGGG34600.024.30717525-29
CGTATCA2451.9039544E-623.6827932
TGCGGCT4307.094059E-1121.91737145
GTCGGTA708.7484295E-820.71498190-94
TACATGG116800.020.0380920-24
TCTCGCA3700.019.98805870-74
Produced by FastQC (version 0.11.5)