FastQCFastQC Report
Sun 19 Mar 2023
SRR4895720_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895720_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517303
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG517900.0144.030232
ACATCGT213200.0143.825293
ACATCGG141250.0143.686843
ACATCGA138600.0143.677083
CATCGGT57900.0143.450154
CATCGTG64450.0143.302324
AAACATC520900.0143.200711
CATCGTA49500.0143.177694
CATCGAT57350.0143.065834
CATCGTT97750.0142.943394
ATCGAGT15800.0142.828315
CATCGAG44200.0142.566094
ACATCGC26150.0142.267293
ATCGTGA16500.0142.012485
ATCGTGT24650.0141.857365
TCGATAG5300.0141.476586
CATCGGA31950.0141.263694
ATCGGTG17300.0140.863245
ATCGTTG25150.0140.757195
ATCGATA14600.0140.740485
Produced by FastQC (version 0.11.5)