FastQCFastQC Report
Sun 19 Mar 2023
SRR4895720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517303
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG120592.3311289515042444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49210.9512800041755025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA18100.349891649574814No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG17960.3471853053239591No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12980.2509167741149771No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6250.12081893977030869No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACGCGCTTCAACTTCGGT5600.1082537700341966No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC5370.10380763305064923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT139450.079.860361
GGTATCA140050.079.052298
GTGGTAT141150.078.17946
GTATCAA142150.078.029929
TGGTATC141550.077.856037
AGTGGTA142400.077.594965
CAGTGGT142450.077.5752264
AGCAGTG143250.077.546942
GCAGTGG146100.075.6868063
CGGTCTG1000.072.49642145
CGGAGCC2701.70985E-1029.535582145
ACATCGT1000.006067512629.0041773
AACATCG1701.0293419E-425.591922
CCGCAAG4101.8189894E-1224.754877145
CATGGGG27550.023.79059825-29
CGACCTA1000.023.19885445-49
AAATATA13750.021.617117145
GAAACCG5150.021.39700185-89
GCGCAGG5200.021.19126145-49
CAGGCGG5350.021.14118870-74
Produced by FastQC (version 0.11.5)