FastQCFastQC Report
Sun 19 Mar 2023
SRR4895719_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895719_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718366
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA151.2563885E-4144.225316
ACGCTCG719000.0144.094921
CGCTCGA719200.0144.054862
GCTCGAT253500.0144.02623
GCTCGAA208850.0143.776443
GCTCGAG245950.0143.638923
CTCGAGT84850.0143.54544
CTCGATG87500.0143.401174
CTCGATA75250.0143.362844
CTCGAAT72350.0143.328264
CTCGATT89550.0143.258974
CTCGAAG73900.0143.054324
CTCGAGA72600.0142.83474
TCGAGTA24600.0142.75965
TCGAGAT24450.0142.750615
CTCGAGG87000.0142.567554
CGAATAG7550.0142.315036
TCGATTG31850.0141.961175
TCGATAG26500.0141.77625
TCGAGTT30250.0141.603035
Produced by FastQC (version 0.11.5)