FastQCFastQC Report
Sun 19 Mar 2023
SRR4895719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718366
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG79621.1083486690628455No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49990.6958848275113243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG28790.4007706378085823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC14360.1998981020816687No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11530.16050314185248188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT10900.15173323904527775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC10280.1431025410445372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT135850.098.5212861
GGTATCA137600.097.103468
TGGTATC138450.096.1931157
GTGGTAT138450.096.1407556
AGCAGTG140600.095.038172
GTATCAA140850.094.81149
CAGTGGT140850.094.6635744
AGTGGTA142150.093.791335
GCAGTGG144300.092.299823
ACGCTCG1109.950418E-846.1384931
CGCTCGA1201.9716936E-742.2936172
CGGTCTG1251.2641931E-534.801605145
AAATATA16000.028.548193145
GCTCGAG1855.8110654E-627.4336973
CGACCTA2600.027.32628345-49
CTCGAGT1358.0783083E-426.853094
TGCGGCT3057.0940587E-1026.148746145
TACGCAC2800.024.85655850-54
CATGGGG37300.023.59694725-29
GAAACCG6350.023.29079285-89
Produced by FastQC (version 0.11.5)