FastQCFastQC Report
Sun 19 Mar 2023
SRR4895718_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895718_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928214
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT31580.3402232674792666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCCA933350.0143.41321
CTCCAAT342750.0142.90463
CTCCAAG309400.0142.624193
CCTCCAA939450.0142.481982
TCCAAGT110550.0141.208634
TCCAATG125550.0140.763054
CCAATAG39500.0140.375495
CTCCAAA276450.0139.540183
TCCAATA104250.0139.436524
TCCAAGA92650.0139.305954
TCCAAGG109800.0138.364364
CCAATGT49750.0138.121695
CCAATTG41450.0137.250695
CCAAGTA34700.0136.936135
CCAAGAT32100.0135.223925
TCCAATT120350.0135.162164
CCAAGTG36350.0134.489185
TCCAAAG100900.0134.275794
CCAATGG39500.0133.98655
CAATAGT14900.0133.078696
Produced by FastQC (version 0.11.5)