FastQCFastQC Report
Sun 19 Mar 2023
SRR4895718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928214
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG167551.805079432113715No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77270.8324588941774202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG66300.7142749409080233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC35120.3783610245051249No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA25940.27946141730247553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC14500.15621397651834598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT200350.085.23511
GTGGTAT202350.083.90876
GGTATCA204100.083.686548
GTATCAA206750.082.7496959
AGCAGTG206000.082.7035142
TGGTATC206550.082.20257
CAGTGGT207100.082.054214
AGTGGTA211900.080.1270755
GCAGTGG213500.079.59453
AAATATA27650.038.547157145
CGGTCTG2401.8189894E-1236.252678145
TACGCAC3250.026.3224750-54
GAAACCG14250.024.21672685-89
AAACCGC14200.024.09777685-89
CGACCTA3250.024.09149445-49
CATGGGG49300.023.7654425-29
TCTCGCA3400.023.4571170-74
TGACGAC3450.023.11516240-44
CGCAGAT15250.023.10513190-94
GCGCAGG15250.022.0582745-49
Produced by FastQC (version 0.11.5)