FastQCFastQC Report
Sun 19 Mar 2023
SRR4895717_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895717_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543964
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGAT195850.0143.880623
CAGCAGA545350.0143.841772
ACAGCAG545350.0143.841771
GCAGATG65150.0143.54794
GCAGATA61650.0143.276294
GCAGATT67100.0142.922444
GCAGAGT61550.0142.806174
AGCAGAG177450.0142.749133
AGCAGAA163700.0142.361973
GCAGAAT59050.0141.647664
CAGAGTG19400.0140.495165
GCAGAAG57550.0140.45324
CAGATAG22050.0140.287835
CAGAGAT17550.0140.103365
GCAGAGA54100.0140.08024
CAGATGG23850.0139.979325
CAGAGTT19850.0139.852925
CAGATTG22350.0139.695275
CAGAAGT18550.0139.547425
CAGATGA18500.0139.534825
Produced by FastQC (version 0.11.5)