FastQCFastQC Report
Sun 19 Mar 2023
SRR4895717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543964
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG113782.0916825378150024No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55991.0292960563566707No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG26570.48845144163952026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17420.3202417807060762No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC13750.25277408063768925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT163000.093.721671
GGTATCA165750.091.939548
GTATCAA167050.091.3542569
CAGTGGT166850.091.167984
AGCAGTG167250.091.1233442
GTGGTAT166950.091.018156
TGGTATC168200.090.384837
AGTGGTA170450.089.242465
GCAGTGG170700.089.026823
CGATCGC400.006050226754.373147
AAATATA14450.027.092499145
CGACCTA651.4879333E-924.53762245-49
GAAACCG6200.023.15328485-89
CATGGGG25950.023.13343225-29
ATGGGGG8550.023.06481225-29
CACGGGA1902.1918905E-422.893953145
AAACCGC6150.022.86997885-89
GCGCAGG6450.022.4798545-49
CGCAGAT6600.022.40832390-94
TACGCAC806.91216E-1021.74925650-54
Produced by FastQC (version 0.11.5)