FastQCFastQC Report
Sun 19 Mar 2023
SRR4895716_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895716_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935009
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA942350.0143.074231
CAGAAGT112350.0143.04384
CAGAATG118100.0142.855834
CACAGAA944050.0142.816592
ACAGAAT341900.0142.743873
ACAGAAG316550.0141.761833
CAGAATT113200.0140.886934
CAGAATA108950.0140.095984
CAGAAGG115150.0139.753364
CAGAAAT101550.0138.732014
CAGAAAG100550.0136.454774
CAGAAGA89800.0135.045994
ACAGAAA286550.0132.951873
AGAATAG41400.0129.221765
AGAAGTA39250.0128.033975
AGAAGTG37400.0127.61995
AGAATGG42350.0127.0040055
AGAATTG40600.0126.2628565
GAAGTAG12250.0125.9533546
AGAATGA38600.0125.333525
Produced by FastQC (version 0.11.5)