FastQCFastQC Report
Sun 19 Mar 2023
SRR4895716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935009
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG143801.5379531106117694No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83610.8942159915038251No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA22720.24299231344297223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG20040.2143294877375512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT13150.14064035747249493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT248150.0100.075711
GGTATCA248250.099.902548
GTGGTAT247000.099.76246
GTATCAA250550.099.101199
TGGTATC249750.098.896147
AGCAGTG250750.098.801422
AGTGGTA250300.098.447125
CAGTGGT250900.098.279994
GCAGTGG258850.095.373583
CCGACGC604.9463374E-448.330513145
CACGCGG700.001056908941.42616145
CGGTCTG2207.4889067E-726.3621145
CCGCAAG6150.024.754656145
CGACCTA2050.024.04943845-49
CGATAGG551.9709114E-723.72842835-39
CATGGGG60950.023.71901525-29
ACATGGG230350.021.35781920-24
TACATGG239750.021.30665620-24
CGCAAGG4451.1459633E-1021.178543145
ATGGGAT38550.021.1391325-29
Produced by FastQC (version 0.11.5)