FastQCFastQC Report
Sun 19 Mar 2023
SRR4895715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679274
Sequences flagged as poor quality0
Sequence length151
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACA680550.0143.903092
AAGGTAC681250.0143.755231
GGTACAG224400.0143.5363
GGTACAT244850.0143.236363
GTACAGT81100.0143.111834
GGTACAA202550.0143.0043
GTACATG79100.0142.44694
GTACAGG75800.0142.276414
GTACAGA67000.0142.241794
GTACATA78400.0141.78784
GTACAAG69500.0141.585364
GTACATT86800.0141.437794
GTACAAT75200.0141.110894
TACAGTG26800.0139.33675
TACAGGT25650.0137.714375
TACAGAG21550.0137.488085
TACAGTA25250.0137.326465
TACATAG26300.0137.05185
TACAGTT28700.0136.894235
TACATGG26400.0136.532675
Produced by FastQC (version 0.11.5)