FastQCFastQC Report
Sun 19 Mar 2023
SRR4895715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679274
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83641.231314609421235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66500.9789863884088013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12630.18593380579854374No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8570.12616411050621693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT122100.091.095391
AGTGGTA121900.090.729565
GGTATCA122650.090.4570858
GTGGTAT123400.089.6134956
GTATCAA125100.089.0716259
TGGTATC124450.088.973947
CAGTGGT125200.088.396064
AGCAGTG125650.088.368072
GCAGTGG129000.086.017013
CGGTCTG2050.042.440662145
CATGGGG32400.023.0480525-29
CGACCTA1300.022.30691145-49
AGGTACA3406.4012056E-821.3306332
ATGGGGT6000.021.02519225-29
TACGCAC1600.020.84302150-54
CGCGAAA1550.020.57992690-94
TGCGGCT5403.6379788E-1220.139666145
TACATGG117950.019.51000420-24
GGGTACG456.871898E-419.3335125-29
GTTCTCG1650.019.332657110-114
Produced by FastQC (version 0.11.5)