FastQCFastQC Report
Sun 19 Mar 2023
SRR4895714_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895714_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530908
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAT190500.0143.848423
AGACGGA532250.0143.823292
AAGACGG532950.0143.63441
ACGGATT63500.0143.053674
ACGGATA61900.0143.024324
GACGGAA148700.0142.976943
ACGGATG64200.0142.841464
GACGGAG186350.0142.757583
CGGATGA17400.0142.117345
CGGATAG20700.0142.099325
ACGGAGT65500.0142.097734
CGGAGTA19950.0142.020775
CGGAGAT19300.0141.947745
ACGGAAT56150.0141.877884
CGGAATG19100.0141.546815
CGGATGT20800.0141.416155
CGGATTA20500.0141.375565
CGGATTG21450.0140.491855
ACGGAGA57100.0140.401214
CGGATAT20250.0139.916755
Produced by FastQC (version 0.11.5)