FastQCFastQC Report
Sun 19 Mar 2023
SRR4895714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530908
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG53321.0043171321584907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50450.9502588019016477No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8210.15464072871382611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT97700.099.893071
AGTGGTA97300.099.549155
GGTATCA97750.098.942538
GTGGTAT98350.098.191486
TGGTATC99100.097.521517
GTATCAA99900.096.804029
CAGTGGT100200.096.677094
AGCAGTG101050.096.5096742
GCAGTGG102900.094.351773
CGGTCTG1900.061.053467145
CGGAGCC1453.487018E-530.000408145
CCGGACG1658.410489E-526.363995145
CGGCGGT1800.024.97303435-39
CGCGAAA1300.024.53833290-94
GTTCTCG1400.023.81951110-114
CATGGGG32300.023.30083525-29
TACGCAC1850.022.727950-54
CGACCTA2000.021.74865745-49
CCTCGTT1500.021.26735735-39
TACATGG95200.021.03363220-24
Produced by FastQC (version 0.11.5)