FastQCFastQC Report
Sun 19 Mar 2023
SRR4895713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619716
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA620250.0144.07442
CGCTTAA185850.0143.814333
CGCTTAG209750.0143.789783
AACGCTT622150.0143.634411
CGCTTAT214300.0143.529393
GCTTAAG65650.0143.323654
GCTTAGG75300.0142.57454
GCTTATG75250.0142.381774
GCTTAGT72000.0141.999184
GCTTAAT67100.0141.62344
GCTTATA66150.0141.259384
GCTTAGA62900.0140.878054
GCTTATT74000.0140.597214
CTTATGG26150.0138.412125
CTTAGGT26000.0138.378715
CTTAGGA21550.0138.179925
CTTAGTA23700.0138.117785
GCTTAAA55600.0137.199654
CTTAGTG23300.0136.775545
CTTATAG23200.0136.121985
Produced by FastQC (version 0.11.5)